Ammonium Chloride Loading Test
The test starts first thing in the morning. The patient should be fasting and has previously been
weighed to allow calculation of the ammonium chloride dose. Patient should drink 200 ml of water
per hour while the test is in progress. Normal diet should be maintained during the test.
06 00 hour: ask patient to drink.
07 00 hour: empty the bladder and discard the urine.
A dose 0.1g/kg body weight of Ammonium chloride is given orally
in gelatin capsules (not entericcoated capsules or tablets).
The whole dose should be taken between 07 00 and 08 00 hours.
If vomiting occurs, this must be reported, and the test may have to be abandoned.
09 - 1500 hour: The patient empties the bladder completely at hourly intervals each time into a separate urine container, label and immediately capped and sent to the lab without delay.
Collect samples as follows:
Test Name Specimen Type
Urinary pH , 09 00 Hour Urine
Urinary pH, 10 00 Hour Urine
Urinary pH, 11 00 Hour Urine
Urinary pH, 12 00 Hour Urine
Urinary pH, 13 00 Hour Urine
Urinary pH, 14 00 Hour Urine
Urinary pH, 15 00 Hour Urine
Urinary pH, 16 00 Hour Urine Container Type : Universal container Volume: 20 ml